APPENDIX

Procedure to create a series of montaged images at varying angles of tilt by using the OASIS.EXE program (method 1)

  1. Start the AutoMontage program, generate a live image, and disable the z-stepper control.
  2. Start the OASIS.EXE program, position the specimen as desired by driving x- and y-tilt motors with mouse clicks.
  3. Return to AutoMontage (click into the image), and activate the z-stepper control. Generate a stack of images, align them, make a montage image, and save the images to disk. Thereafter disable the z-stepper control.
  4. Repeat steps 2 to 4 with the next following tilting positions.