Overcoming sampling issues in dental tribology: Insights from an experimentation on sheep
Article number: 20.3.53A
https://doi.org/10.26879/762
Copyright Palaeontological Association, October 2017
Author biographies
Plain-language and multi-lingual abstracts
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Submission: 15 February 2017. Acceptance: 2 October 2017
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ABSTRACT
Dental microwear has been used in paleoecology for nearly half a century. The advent of technologies in the past decade has allowed for enamel surfaces to be scanned in 3D, and thus for microwear textures to be characterized as a whole. Although dental microwear texture analysis is widely used, few studies have tackled the issue of sample representativity, or the variability of the microwear signal along a facet or a tooth row. How much information can we accurately extract from a fossil sample when tooth wear is considered? Can we accurately make inferences on a whole species based on fossil samples primarily comprised of isolated teeth? These matters remain to be characterized in a controlled setting. In this study, we tackle this issue from the ground up. A large-scale controlled food trial conducted on domestic sheep provides the framework in which to test these fundamental questions. Our results highlight that analyzing a 200 × 200 µm surface allows for better differentiation between dietary categories, as opposed to analyzing smaller surfaces. Comparisons of facets from upper and lower molars reveal significant variations depending on the contribution to either the buccal or the lingual shearing phase I during the chewing cycle. Investigating the microwear signal along the tooth row does not reveal any significant variation between molars belonging to a same tooth row. However, when simulating three fossil samples composed of isolated upper or lower molars from M1 to M3 from the three sets of ewes, our study does highlight poor results in discriminating dietary categories.
Anusha Ramdarshan. Institut de Paléoprimatologie et Paléontologie Humaine: Evolution et Paléoenvironnements UMR 7262 (CNRS and Université de Poitiers), 86073 Poitiers Cedex 9, France. anusha.ramdarshan@univ-poitiers.fr
Cécile Blondel. Institut de Paléoprimatologie et Paléontologie Humaine: Evolution et Paléoenvironnements UMR 7262 (CNRS and Université de Poitiers), 86073 Poitiers Cedex 9, France. cecile.blondel@univ-poitiers.fr
Denis Gautier. Ferme du Mourier, Institut de l’Elevage, 87800 St Priest Ligoure, France; Centre Interrégional d’Information et de Recherche en Production Ovine, Ferme du Mourier, 87800 Saint Priest Ligoure, France. Denis.Gautier@idele.fr
Jérôme Surault. Institut de Paléoprimatologie et Paléontologie Humaine: Evolution et Paléoenvironnements UMR 7262 (CNRS and Université de Poitiers), 86073 Poitiers Cedex 9, France. jerome.surault@univ-poitiers.fr
Gildas Merceron. Institut de Paléoprimatologie et Paléontologie Humaine: Evolution et Paléoenvironnements UMR 7262 (CNRS and Université de Poitiers), 86073 Poitiers Cedex 9, France. gildas.merceron@univ-poitiers.fr
Keywords: Dental microwear texture analysis; representativity; mammals; diet; sheep; controlled-food-testing
Final citation: Ramdarshan, Anusha, Blondel, Cécile, Gautier, Denis, Surault, Jérôme, and Merceron, Gildas. 2017. Overcoming sampling issues in dental tribology: Insights from an experimentation on sheep. Palaeontologia Electronica 19.3.53A: 1-19. https://doi.org/10.26879/762
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Copyright: October 2017 Palaeontology Association.
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INTRODUCTION
The fossil record suffers from its fragmentary nature. The conditions necessary for fossilization are very rarely met, and consequently the fossil record only represents a partial representation of past biodiversity (Foote and Sepkoski, 1999; Benton et al., 2000; Le Fur et al., 2011). Inferences in paleontology, but also disciplines such as paleoecology and paleoanthropology rely on fossil specimens, which can vary in number for a given species from thousands of cranial and post cranial remains to a single isolated tooth. Paleoecology is particularly dependent on this issue. Ecological traits, from diet to ranging behavior, vary between species but also within a given species from population to population. For example, both red deer and roe deer vary significantly in the diet throughout the year (Storms et al., 2008). Some taxa even vary from being grazers for a given season to incorporating a substantial amount of fruit in its diet for another (Gagnon and Chew, 2000). Environment can also be a significant factor influencing diet. For example, impalas (Aepyceros melampus) living in open savanna and grassland landscapes generally eat more grass than their counterparts in savanna woodlands (Codron et al., 2005). Local events with high impact on vegetal resources may also be a major source of dietary variation. For example, in South America, annual flooding forces the red brocket deer (Mazama americana) and the collared peccary (Tayassu tajacu) to retreat to floodplain islands and to shift from a frugivorous to a woody browse diet (Bodmer, 1990). Interpretations in the fossil record are based on specimens which, although belonging to the same locality and possibly the same species, offer no guarantee of originating from the same season or even the same year (or even century) of death. In this context, making inferences on a whole species based on a few specimens seems challenging at best.
Among the available paleoecological methods, the analyses of tooth wear at different scales are robust proxies to interpret dietary habits of extinct species in the fossil record. There are two main types of tooth wear, both resulting in the loss of small fragments of enamel from the tooth itself: attrition, the wear resulting from tooth-to-tooth contact, and abrasion, resulting from food-to-tooth contact (Kaiser et al., 2013). Although tooth wear can also have other root causes such as chemical erosion, abrasion is of particular interest in paleoecology as it varies according to the physical properties of the food ingested over the last weeks of an animal’s life (Calandra and Merceron, 2016; Merceron et al., 2016; Ramdarshan et al., 2016). Dental microwear texture analysis (DMTA) has now become a widespread tool for reconstructing the past diet of mammals (Ungar, 2015; Calandra and Merceron, 2016; DeSantis, 2016) and even other vertebrates (Purnell et al., 2007; Gill et al., 2014). However, although this method is widely used, few studies have tackled the issue of sample representativity. Can a single scan, representing at best a few hundred square micrometers, give an accurate representation of a whole dental facet, let alone a whole tooth? How does the microwear signal vary along a facet? How large does a scan have to be to provide accurate discrimination between different diets? Answers to these questions are fundamental before reliable dietary interpretations can be given. Microwear is now widely used in paleoecology to reconstruct past diets. However, most studies use whatever material is available: upper, lower, first, second, third molars, and even sometimes premolars are often jumbled together in the same analysis in order to maximize sample size. However, several factors vary along the tooth row. For example, the force of the power stroke is known to vary along the tooth row (Pérez-Barbería and Gordon, 1998, 1999; Radinsky, 1985; von Koenigswald et al., 2013). Efficiency in food comminution depends directly on molar surface occlusal area and contact area (Pérez-Barbería and Gordon, 1998), which varies according to the tooth considered. All foods are not reduced in the same way along the tooth row (Reilly et al., 2001). Furthermore, previous studies (Schulz et al., 2010; Taylor et al., 2013, 2016) have highlighted inter-tooth differences in several taxa. In these conditions, are the microwear patterns on the first, second and third molars along a same tooth row directly comparable?
These matters remain to be characterized in a controlled setting. In this study, we tackle this issue from the ground up. A large-scale controlled food trial conducted on domestic sheep provides the framework in which to test these fundamental questions. Controlling the physical and mechanical properties of the diet (i.e., toughness, hardness) allows us to characterize the microwear signal. Here, we focus on the questions of scan size representativity, signal variation along the tooth row and between upper and lower cheekteeth (Figure 1).
MATERIAL AND METHODS
Controlled-food Trials
The controlled-food trials were carried out at the Mourier farm (Limousin region, France; agreement number B-87-176-01), under the supervision of the Centre Interrégional d’Information et de Recherche en Production Ovine (CIIRPO) and the Institut de l'Elevage (Idele; see details in Merceron et al., 2016; Ramdarshan et al., 2016). G.M. and D.G. who have official approval to carry out such procedures, designed these trials. They were performed on domestic sheep (Ovis aries), using only ewes from the Vendéen breed. All experiments were conducted on cull ewes, meaning sheep no longer suitable for breeding and sold for meat. None of the experiments required the sheep to be handled. In this study, all the ewes had been kept in the same pasture highly dominated in grasses for six months before the start of the trials. As such, their dental microwear textures are considered to be homogenous at the beginning of the controlled food testing. Sheep had full access to foods with which they were familiar. The ewes were kept inside a covered sheepfold. The sheep were not kept on hay, which they would have eaten, but on dust-free wood shavings used in equestrian centres. Feeding troughs were covered with a plastic film and cleaned out daily to avoid contamination. None of the ewes lost weight during the experiments (see details in Merceron et al., 2016 and in Ramdarshan et al., 2016).
Thirty sheep were included in this study (Table 1), divided into three groups of ten. Three groups were each given foods with different physical and mechanical properties: soft and silica-depleted browse, tough and silica bearing grass, hard and brittle seeds in complement with soft browse. These qualitative terms are used to broadly qualify the food properties used in this study. Grasses are considered tougher, i.e., to have a higher energy release rate (Berthaume, 2016; Thiery et al., 2017) than browse such as clover. However, we should note that the clover and grass assemblages foraged by the ewes during the experiments do not display any significant differences in fracture toughness (meaning the ability to absorb deformation energy per unit volume before failure) measured thanks to a tensile test (Merceron et al., 2016). Seeds are qualified as hard and brittle as comminution can be obtained through stress created between flat surfaces, as opposed to grasses and browse which require blades to cut through them (Fortelius, 1985). For details on the specific properties of these foods (specific abundance, bio-silica content, toughness, hardness), refer to Merceron et al. (2016) and Ramdarshan et al. (2016). The first group of cull ewes was fed on a red clover-dominated silage (~1.650 kg per ewe and per day), the second on a multispecific assemblage highly dominated by grasses (~1.550 kg per ewe and per day), and the third on the clover-dominated silage (~1.275 kg per ewe and per day) in addition with barley kernels (~0.425 kg per ewe and per day). The fodders were harvested and then bale-wrapped 24 hours after the cutting in order to guarantee similar natural physical properties (percentage of dry matter about 50%) to the uncut plant throughout the controlled food testing. The ewes had full access to the food. These amounts were defined by giving large amounts of fodder and measuring how much the ewes had consumed in 24 hours. It is worth highlighting that silage tends to be acid and one could hypothesize that differences in dental microwear textures reflects acid erosion rather abrasion or attrition. However, it is known that saliva plays an active role in protecting dental enamel from acid erosion (Hannig and Balz, 1999; Lendenmann et al., 2000; Wetton et al., 2006).
Casting and Data Analysis
The skulls are housed at the iPHEP lab (UMR 7262 CNRS and Université de Poitiers). Each analyzed tooth (first, second, and third upper molars [UM1, UM2, and UM3, respectively] and first, second, and third lower molars [lm1, lm2, and lm3, respectively]; Figure 1.1) was then carefully cleaned with a cotton-swab soaked in acetone. Molds are then made using a polyvinylsiloxane elastomer (Regular Body President, ref 6015 - ISO 4823, medium consistency, polyvinylsiloxane addition type; Coltene Whaledent). This product is known to be the most efficient one to replicate a given surface (Galbany et al., 2006; Goodall et al., 2015).
The molds are then placed under a Leica DCM8 confocal profilometer using white light confocal technology with a Leica 100× objective (Numerical aperture = 0.90; working distance = 0.9 mm). The lateral resolution is an (x, y) interval of 0.129 μm, with a vertical numerical step of 1 nm.
The analyses were performed using the Scale-Sensitive Fractal Analysis using Toothfrax and Sfrax software (Surfract, www.surfract.com) following Scott et al. (2006). Complexity (Asfc or Area-scale fractal complexity) is a measure of the roughness at a given scale. Anisotropy (epLsar or exact proportion of length-scale anisotropy of relief) measures the orientation concentration of surface roughness (Table 1; Appendix 1).
Before each sets of statistical tests in this study, data were rank-transformed in order to ensure the conditions for parametric tests were met (Conover and Iman, 1981). Data were then analyzed using two-way repeated measures ANOVAs and corresponding post-hoc tests (Pairwise Student test, performed with the Bonferroni adjustment method to take into account non-independent observations).
Different Case Studies for Different Questions
Differences in scan size. Dental microwear texture analysis has been highly improved by Scott et al. (2005, 2006). Since then the method has been widely used to reconstruct diet in both living and extinct mammals. The standard scan size of 4 adjoining scans of 100 × 140 µm was defined in order to match the size used in early studies using SEM. Several studies, carried out on smaller taxa such as rodents or lagomorphs, even use scans as small as 15 × 15 µm (Schulz et al., 2013; Withnell and Ungar, 2014; Burgman et al., 2016; Calandra et al., 2016a, 2016b). Here, we test the representativity of scan size (Figure 1.2). A scan was performed at the central position for each disto-labial protoconid facet of the second lower molar. From this scan several surfaces were treated: 50 × 50 µm, 100 × 100 µm, and 200 × 200 µm. Each 200 × 200 µm scan was also treated as four equal subsurfaces, to test the pertinence to analyze a surface as a whole, or as a combination of subsurfaces as established by Scott et al. (2005, 2006). An additional value, called 3-spot mean, corresponds to the mean of the anisotropy (epLsar) and complexity (Asfc) of three different scans along the facet (i.e., in a buccal, central and lingual position). Three scans along the facet were made in order to test the variation of the microwear signal. Although variations can be seen between the lingual, central, and jugal positions, no significant differences are highlighted by this study (see Appendix 2, Appendix 3). The mean of the three values (buccal, central, and lingual) is also used to test differences with value gained from scans of different size.
Differences between upper and lower teeth. Numerous microwear studies use both upper and lower molars in order to maximize sample size. The lingual paracone facets and the labial protoconid facets are traditionally used in dental microwear studies (Semprebon et al., 2004; Merceron et al., 2012; Scott, 2012; Rivals et al., 2015) as they occlude during the shearing phase I (Janis, 1979, 1990) and supposedly share similar microwear patterns. Phase I is dominated by shearing actions as opposed to phase II during which grinding actions are more prevalent (Crompton and Hiiemae, 1969; Janis, 1990; Lucas, 2004). Here, we suppose that the paracone first enters in to contact with lingual cuspids on the lower molar at the beginning of phase I, and then with the buccal cuspids as the lower molars move centrally. It results that the facet on paracone traditionally used in microwear studies enters in contact with more enamel facets than the buccal dental facets of the protoconid on lower molars. Here we test the homogeneity of the dental microwear texture between upper and lower second molars issued from the very same individual (Figure 1.2). Alternatively, we also consider lingual protocone facets on upper second molars because in the same way as the buccal facets of the protoconid on lower molars, they occlude with fewer enamel facets from the homologous counterparts (Figure 1.3). All of the comparisons are made with 200×200 µm scans performed on the central position along the enamel band.
Differences between cheekteeth along the tooth row. Similarly to upper and lower molars, numerous microwear studies jumble first, second, and third molars together in order to maximize sample size (Merceron et al., 2005a, 2005b, 2012; Ungar et al., 2007, 2016; García-González et al., 2015). Here, we test the homogeneity of the microwear signal along the lower tooth row (Figure 1.4). Each disto-labial protoconid facet of the first, second, and third lower molars was scanned for each ewe. Dental microwear texture analysis is performed on 200×200 µm surfaces.
Simulation as Bridge Between Experimentation and Fossil Samples
In this study, we will test what the signal carried by a fossil sample really means. Most microwear studies utilize associated dentitions where the specific locus of the tooth sampled is known and pseudoreplication of individuals is absent or minimal (Scott, 2012; Scott et al., 2012; Merceron et al., 2014). However, many have to contend with isolated teeth implying that one individual could be represented several times in one sample (Merceron et al., 2012; Tütken et al., 2013; Oliver Pérez et al., 2014). Does regrouping first, second, and third lower with upper molars as paleontologists used to do reinforce or weaken ecological interpretations compared with analysis run on a single tooth type (e.g., second lower molar)? To answer this question, each disto-labial protoconid facet on lower molars or each mesio-lingual paracone facet on upper molars were scanned (Figure 1). Complexity (Asfc) and Anisotropy (epLsar) were calculated for each scan. This results in a dataset with 60 observations (UM1-3 and lm1-3) for a 10-ewe sample. The ewes considered in this study have different ages, and so some individuals do not have a fully erupted third molar. However, they are considered in the datasets so the observation they represent is associated with no values for the two textural parameters.
In order to simulate samples with isolated cheekteeth belonging to possibly the same individuals, three sets of 10, 20, or 30 observations are drawn randomly from each 60-observation sample (grass, clover, and clover/barley; Figure 1.5). This process is repeated a thousand times to generate a significant amount out of possible cases. As studies on fossil specimens have to primarily contend with isolated teeth with no indication as to whether or not they belong to the same individual, no precaution is taken here to avoid sampling multiple observations from the same individual (although the random sampling is without replacement). The result is a dataset with three groups (grass, clover, and clover/barley) containing 10, 20, or 30 observations each.
For each of the thousand iterations, data are rank transformed to ensure the conditions to carry out parametric statistical tests are met. As we are considering datasets as we would in the fossil sample, single classification ANOVAs (and not repeated measures) are then performed to highlight any significant differences between clover-, grass-, and clover/barley-fed ewes. Results are reported as the frequency of significant p -values over the 1000 iterations.
RESULTS AND DISCUSSION
Scan Mode and Size on Lower Molar
The two-way repeated measure ANOVA shows significant variations in complexity (Asfc) between scan options (marginally with p = 0.054; Table 2) with a significant effect of the interaction between scan option and dietary groups (Figure 2). The two-way repeated measure ANOVA also shows marginal variations in anisotropy (epLsar) between dietary groups. The pairwise post-hoc comparisons highlight significant differences in complexity (Asfc) and anisotropy (epLsar) between the three different dietary groups for all scan size options except for the 3-spot mean (Table 1 and Table 3; Figure 2). Indeed, there is no significant difference between the different dietary groups of ewes when several scans at different position along a facet are considered. To multiply scans over a dental facet, instead of focusing on a scan at the central position, blurs the differences between groups.
When looking at the different scans from 200 to 50 µm a side, differences between diets depend on which scan size is considered. Results at 200 µm, 100 µm, and 50 µm all show significant differences between the clover-fed and grass-fed samples for Asfc (Table 3). None highlight differences in complexity (Asfc, Table 2 and Table 3, Figure 2) between the clover/barley-fed ewes and the two other groups. Using the 4-subsurface median option, differences in complexity are significant between clover-fed and grass-fed ewes, but again none between the clover/barley fed ewes and the two other groups (Table 3).
Results for anisotropy (epLsar) are more varied. Results at 50 µm do not show any significant differences between groups (Table 3). This result may seem counterintuitive given the very different means of each group. Actually, the high inter-individual differences in anisotropy (and complexity as well) due to the random occurrence of a given microwear feature (such as deep overlapping pits with complex slopes) and thus high variance (Figure 2) in each group is such that there is a major overlap between all the groups, preventing the repeated measures ANOVA from highlighting any potential difference in microwear textures. This result would seem to indicate that looking through a 50 µm window for large mammals is indeed not wide enough to be able to see the whole picture. At 100 µm a side, only the clover/barley-fed ewes show any significant difference in anisotropy (epLsar) from the grass-fed ewes (Table 1 and Table 3). No difference between clover and grass fed ewes are found at this scale. Both the epLsar at 200 µm a side and the 4-subsurface median option show differences between clover/barley and grass fed ewes, and between clover and grass fed ewes. Furthermore, using the traditional median of four adjoining scans does not provide any additional information compared to using a 200×200 µm square. As such, a 200×200 µm seems more pertinent for future dental microwear texture analysis. This study, carried out on domestic sheep, does carry implications for other taxa, notably in ruminants. When considering applications beyond the model organism, other factors need to be taken into account. A 200 x 200 µm surface might not be available in some taxa, or differences in masticatory cycles might produce peculiar microwear patterns in others. However, these results suggest that scan size can be a limiting factor when trying to highlight differences in diet.
Differences Between Facets from Upper and Lower Teeth
The two-way repeated measures ANOVA highlights significant variations in Asfc between upper and lower molars when the disto-labial protoconid and mesio-lingual paracone facets are considered (Table 1 and Table 4). Disto-labial protoconid facets show more complex surfaces than the mesio-lingual paracone ones (Table 1, Table 4, Table 5, Figure 3). Dietary differences between the ewe samples are more pronounced when considering the disto-labial protoconid facets of the lower molars (Table 1, Table 4, Table 6; Figure 3).
When using a repeated measures ANOVA, there is no significant variation in Asfc when the disto-labial protoconid and mesio-lingual protocone facets are considered (Table 1 and Table 7; Figure 3). The repeated measures ANOVA does highlight significant variations in epLsar between the different dietary groups and between facets. However, further analysis pinpointing the latter differences show that they are not between upper and lower molars of the same dietary category (Table 8).
These results highlight the importance in dental microwear analysis to pinpoint dental facets at a broad scale and to consider their precise function(s) that intervene during the masticatory cycle (buccal and lingual shearing in phase I) before running any micrometric-scale analysis.
Differences Between Cheekteeth Along the Tooth Row
A two-way repeated measures ANOVA was carried out on first, second, and third lower molars in order to highlight potential differences between groups (Table 9, Figure 4). However, this analysis fails to show any significant variations in complexity (Asfc), suggesting that there is minimal variation along the tooth row. The study does highlight differences in anisotropy (epLsar). However, further investigation using post-hoc multicomparison tests reveal that first, second, and third molars of the same dietary category do not differ significantly from one another (Table 10). The only differences shown by the post-hoc tests are in fact between different dietary categories (Table 10). Again, this result suggests that there is little variation in the microwear textures along the tooth row in a given dietary category.
The results in this study fail to highlight differences in microwear texture between the different cheek teeth on the lower left jaw. This would seem to indicate that microwear textures are relatively homogenous along the tooth row. Simply put, microwear is a direct reflection of how food is reduced. This simple notion means that microwear depends on numerous factors such as the size, shape, and material properties of food particles, the size, shape, material properties of teeth, occlusal forces, vectors, and more (Lucas, 2004). In these conditions one expectation would be that microwear textures show significant differences between different teeth from the same tooth row. Our results indicate this is not the case. The lack of variations in dental microwear textures along the tooth row could mean that the power stroke, even if maximum at a given point along the tooth row, would see its force homogenously distributed due to the tooth row acting as a geometric plane (in ruminants compared to carnivorous mammals with molar shape heterogeneity).
Simulating a Fossil Sample
The lack of any significant differences between teeth from the same tooth row suggests that dietary differences will still be able to be highlighted when using first, second, and third molars together. We have also highlighted the misuse of the combination of upper and lower dental facets, assumed to be homologous (or antagonist sensu Schultz et al., 2017) because occluding during the dental shearing phase I (Janis, 1990), but actually involved in different ways during the phase I. Thus, here, we investigated the effects of grouping altogether these two types of dental facets from upper and lower molars, a habit widely used by paleoecologists to increase sample size and supposedly strengthen their analysis.
To test this hypothesis, we simulated fossil samples using a random sampling (see Material and Methods; Appendix 4). Results for the simulation of a fossil sample are summarized in Table 11 and Figure 5. These results are detailed in Appendix 5, Appendix 6, Appendix 7, Appendix 8, Appendix 9, Appendix 10. Complexity (Asfc) and anisotropy (epLsar) for each dietary group and for each iteration are presented in Appendix 5, Appendix 6, Appendix 7. The frequency with which significant differences are highlighted vary according to the number of simulated individuals per group. When considering n = 10, significant differences are highlighted in only 7.1% (Asfc) and 25.6% (epLsar) of the 1000 iterations. Frequencies are higher when considering 20 individuals per group (8.7% and 46.6%, respectively) and highest when considering 30 individuals per group (10% and 72.1%, respectively).
Our results suggest that combining dental microwear textures on disto-labial facets on protoconid of first, second, and third lower molars together with the textural parameters from the mesio-lingual facets on upper molars increases the sample variance to the point of hiding any differences between dietary categories. Numerous studies base their microwear results on data including significant amount of isolated, determined, or undetermined teeth, jumbled together to maximize sample size (Ungar et al., 2007; Merceron et al., 2012; Tütken et al., 2013). Our results indicate that this practice does not help gain statistical strength. Such practices could therefore result in artificially inflating the mixed feeding groups to the detriment of the grazing and browsing ends of the dietary spectrum among herbivorous mammals.
Another factor which seems to influence the results is sample size. Indeed, in this simulation, the highest frequency with which significant differences are highlighted is when N = 30. Increasing sample size does seem to reduce variance and therefore the overlap between dietary categories. However, while this did increase efficiency for anisotropy (epLsar), this was not the case for complexity (Asfc). Even when N = 30, differences in Asfc are only significant in 10% of the 1000 iterations. This result would seem to indicate that strengthening sample size by itself is not sufficient, but that it also needs to be combined with a strict protocol focused on a specific tooth locus.
CONCLUSIONS
By controlling the dietary breadth of three different groups of ewes, this study tackles fundamental issues in the field of dental microwear texture analysis such as scan size, differences between teeth of a same tooth row, or differences between differences dental facets from upper and lower teeth. Our results have shown than a scan of 200×200 µm provides the best results in order to discriminate between different dietary categories, and provides the same information as using the median of 4-subsurfaces, as used in many previous studies. Furthermore, results allowed for a direct comparison of microwear textures between teeth from a same tooth row. No significant differences could be shown by this study. Differences were, however, highlighted between previously considered homologous facets in upper and lower teeth, highlighting the importance to split dental facets depending on their function during the chewing cycle, even within the two buccal and lingual shearing phases during phase I. We overcome this issue by identifying dental facets from upper and lower molars that indeed share the same functions during the lingual shearing phase I and thus similar textures on enamel surfaces. Finally, the ewe dataset is used to simulate the characteristics of a fossil sample. We show that the common practices of combining isolated determined and undetermined upper and lower molars (especially using inappropriate dental facets) resulted in a loss of ecological discrimination at a population scale, but also at the level of species and communities through space and time. To track environmental changes in the fossil record requires us to indeed strengthen the protocol rather than the sample size.
ACKNOWLEDGMENTS
The authors thank the staff from the Mourier Farm Station, E. Pottier (IDELE), B. Grillon, J.-M. Grolleau (Limovin), F. Vannier (President of the CIIRPO), J. Merceron (Director of the IDELE), as well as G. Reynaud (iPHEP). The authors thank N. Brunetière and A. Francisco (P prime Institute, CNRS, ENSMA, and University of Poitiers), two members of the TRIDENT project team. We are also grateful to editors of PE and anonymous reviewers that improved the manuscript by all of their comments. This study was financed by the Project ANR TRIDENT (ANR-13-JSV7-0008-01, PI: G. Merceron).
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